IGCSE Biology · Topic 19 · 2026 Exam

Ecology

Ecosystem, population, community, habitat, abiotic and biotic factors; food chains and webs; three types of ecological pyramids; why energy decreases between trophic levels; Extended carbon, water, and nitrogen cycles with processes and organisms; limiting factors; quadrat, transect, and mark-recapture sampling; Extended Lincoln-Petersen formula with assumptions.

Sub-section 19.1 Core Extended Papers 1–4
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Topic 19.1

Ecology

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Ecosystem Key Terms

TermDefinition
EcosystemAll the living organisms in an area together with the non-living (abiotic) components of the environment
PopulationAll the organisms of the same species living in the same area at the same time
CommunityAll the populations of different species living in the same area at the same time (biotic component only)
HabitatThe place where an organism lives, characterised by its physical features and the species present
Abiotic factorsNon-living factors of the environment (temperature, light intensity, water availability, soil pH, wind speed, salinity)
Biotic factorsLiving components of the environment (predation, competition, disease, food availability, parasitism)

Food Chains and Food Webs

Key rules

Food chain: shows the linear transfer of energy from one organism to the next. Arrows point in the direction of energy flow (from prey to predator, from eaten to eater).

Food web: a network of interconnected food chains in an ecosystem. More realistic; shows multiple feeding relationships.

Producers (plants/algae/phytoplankton) are always at the start — they convert light energy to chemical energy by photosynthesis.

Consumers: primary (herbivores) → secondary → tertiary (carnivores).

Decomposers (bacteria and fungi) break down dead organic matter, returning minerals to the soil.

Constructing and interpreting food webs (Paper 5/6)

When asked to predict the effect of removing a species: trace all arrows connected to that species. Removing a prey species → predators above may decrease (less food). Removing a predator → prey below may increase (less predation), which may cause species further below to decrease (more competition for food/over-grazing).

Energy Flow and Ecological Pyramids

Energy enters ecosystems via photosynthesis and is transferred along food chains. At each trophic level, most energy is lost and cannot be passed to the next level.

Type of pyramidWhat it showsAlways upright?
Pyramid of numbersNumber of organisms at each trophic levelNot always — can be inverted (e.g. one tree supporting thousands of insects)
Pyramid of biomassTotal dry mass of organisms at each levelUsually upright; occasionally inverted in aquatic systems (fast-reproducing phytoplankton)
Pyramid of energyEnergy available at each levelAlways upright — energy always decreases at each transfer
Why energy is lost between trophic levels

At each transfer, energy is lost as:

  • Heat from cellular respiration (metabolic processes)
  • Undigested material in faeces (passed to decomposers, not to next consumer)
  • Urine and excretory products (containing chemical energy not absorbed)

Typically only 10–20% of energy at one level is transferred to the next. This limits food chain length to about 4–5 links.

Carbon Cycle — Extended

ProcessDirection of carbon transferOrganisms involved
PhotosynthesisAtmosphere → biospherePlants, algae, cyanobacteria
RespirationBiosphere → atmosphereAll living organisms
CombustionFossil fuels/biomass → atmosphereHuman activity (burning)
DecompositionBiosphere → atmosphere/soilBacteria and fungi (decomposers)
FeedingProducer → consumerHerbivores, carnivores
FossilisationBiosphere → lithosphereGeological processes (over millions of years)
Human impact on the carbon cycle

Burning fossil fuels: releases CO₂ stored for millions of years → rapidly increases atmospheric CO₂ → enhanced greenhouse effect → global warming.

Deforestation: (1) removes trees that absorb CO₂ by photosynthesis; (2) burning or decomposing felled trees releases stored carbon → double impact on atmospheric CO₂.

Water Cycle — Extended

ProcessDescription
EvaporationWater from oceans, lakes, and soil heated by sun → water vapour rises into atmosphere
TranspirationWater vapour released from plant leaves through stomata → into atmosphere
CondensationWater vapour cools as it rises → condenses into water droplets → forms clouds
PrecipitationWater falls as rain, snow, hail, or sleet back to Earth’s surface
Surface runoffWater flows over land into rivers, lakes, and eventually seas
InfiltrationWater soaks into soil → replenishes groundwater (aquifers)

Nitrogen Cycle — Extended

Nitrogen is essential for making amino acids, proteins, and nucleic acids. Atmospheric N₂ cannot be used directly by most organisms — it must be converted to usable forms.

ProcessConversionOrganisms/agents
Biological nitrogen fixationN₂ → ammonia / ammonium compounds (NH₃)Rhizobium in legume root nodules; Azotobacter and other free-living nitrogen-fixing bacteria in soil
Atmospheric nitrogen fixationN₂ → nitrogen oxides → nitrates in soil waterLightning — energy of lightning converts N₂ to nitrogen oxides; these dissolve in rain to form nitrates absorbed by plant roots
NitrificationNH₃ → NO₂⁻ → NO₃⁻ (nitrate)Nitrifying bacteria (Nitrosomonas, Nitrobacter) in aerobic soil
AssimilationNO₃⁻ → organic N (proteins, DNA)Plants absorb nitrate via roots; animals eat plants
AmmonificationOrganic N → NH₃ / NH₄⁺Decomposer bacteria and fungi break down dead organic matter
DenitrificationNO₃⁻ → N₂ (returns to atmosphere)Denitrifying bacteria in waterlogged/anaerobic soils
Why farmers add nitrogen fertilisers

Crops absorb nitrate from soil. Harvesting removes this nitrogen permanently from the field — it is not returned via decomposition as it would be in a natural ecosystem. Over time, soil nitrogen is depleted. Farmers replenish it by adding inorganic nitrogen fertilisers (e.g. ammonium nitrate) or organic fertilisers (manure, compost), or by growing leguminous cover crops that fix atmospheric N₂.

Population Size and Limiting Factors

Population size is determined by the balance between births + immigration and deaths + emigration. In natural ecosystems, populations do not grow indefinitely — they are limited by:

Limiting factorTypeEffect on population
Food availabilityBioticLess food → starvation → increased death rate
PredationBioticMore predators → prey population decreases
DiseaseBioticPathogens spread more easily in dense populations
CompetitionBioticIntraspecific (same species) or interspecific (different species) competition for resources
TemperatureAbioticToo hot/cold reduces survival and reproduction
Water/rainfallAbioticDrought reduces food and habitat; flooding alters habitat

Sampling Techniques

To estimate population size or study species distribution without counting every individual, scientists use sampling methods.

MethodUsed forHow it works
Quadrat Plants and slow-moving/sessile animals A square frame (e.g. 0.5×0.5 m or 1×1 m) placed randomly in the study area. Count the number/percentage cover of species inside. Repeat many times and calculate the mean. Multiply mean by total area to estimate total population.
Transect Distribution along an environmental gradient (e.g. shore, edge of woodland) A line is laid across the area. Record species at regular intervals (belt transect: quadrats placed at intervals along the line). Shows how species distribution changes with the gradient.
Mark-recapture Mobile animals Catch a sample, mark and release. Later, catch a second sample. Count the proportion of marked individuals in the second sample. Lincoln-Petersen formula: N = (n₁ × n₂) ÷ m₂

Mark-Recapture Calculation — Extended

Lincoln-Petersen formula

N = (n₁ × n₂) ÷ m₂

Where: N = estimated total population; n₁ = number caught and marked in first sample; n₂ = total number caught in second sample; m₂ = number of marked individuals in second sample.

Assumptions: (1) marks do not affect survival or behaviour; (2) marks are not lost between captures; (3) the population is closed (no births, deaths, immigration, emigration between samples); (4) marked individuals mix randomly with the population before recapture.

Example: n₁ = 40 marked; n₂ = 25 total recaptured; m₂ = 8 marked in second sample. N = (40 × 25) ÷ 8 = 1000 ÷ 8 = 125.

MCQ · Topic 19.1Core

In an ecosystem, a pyramid of energy is always upright. Why can a pyramid of numbers be inverted?

  • A. Energy can increase as it passes through a food chain
  • B. One large producer organism can support many smaller consumers, so consumer numbers can exceed producer numbers
  • C. Decomposers add energy to the food chain, reversing the pyramid
  • D. The number of organisms always equals the energy available at each level
Answer: B. A single large organism (e.g. one oak tree) can provide food for thousands of caterpillars — so the number of consumers greatly exceeds the number of producers, producing an inverted pyramid of numbers. Energy pyramids can never be inverted because energy always decreases at each trophic transfer — you cannot have more energy at a higher level.
Calculation · Mark-recaptureExtended

A student catches 60 woodlice from a garden, marks them with white paint, and releases them. Two days later, she catches 45 woodlice from the same area and finds 9 are marked. Estimate the total woodlouse population. Show your working. [2 marks]

Mark scheme
  • N = (n₁ × n₂) ÷ m₂ = (60 × 45) ÷ 9 [1 mark for correct substitution]
  • N = 2700 ÷ 9 = 300 woodlice [1 mark]
Exam Prep

Comprehensive Practice Questions

Mixed questions across Topic 19.

MCQ · Energy flowCore

Which of the following is the main reason why energy is lost between trophic levels in a food chain?

  • A. Organisms at higher trophic levels are smaller and need less energy
  • B. Energy is released as heat during cellular respiration and some material is lost as undigested faeces
  • C. Producers convert only a small fraction of sunlight into chemical energy
  • D. Decomposers absorb most of the energy before it reaches consumers
Answer: B. Energy is lost between trophic levels mainly as heat from respiration (the largest loss) and as undigested material in faeces (which goes to decomposers rather than the next consumer). Option C describes photosynthetic efficiency, which is a separate issue from trophic transfer efficiency.
Paper 3 Style · Nitrogen cycle + samplingCore

(a) State the names of two types of bacteria involved in the nitrogen cycle and describe the role of each. [4 marks]
(b) A student uses quadrats to estimate the number of daisy plants in a field of area 200 m². In 10 randomly placed 0.5 m × 0.5 m quadrats, she counts: 3, 5, 2, 4, 3, 6, 2, 4, 5, 6 daisy plants. Estimate the total number of daisies in the field. [3 marks]

Mark scheme
  • (a) Any two of:
    Nitrogen-fixing bacteria (e.g. Rhizobium): convert atmospheric N₂ into ammonia/ammonium ions that plants can use [2 marks]
    Nitrifying bacteria (e.g. Nitrosomonas/Nitrobacter): convert ammonia to nitrite, then nitrate in the soil [2 marks]
    Denitrifying bacteria: convert nitrate back to N₂ gas, returning nitrogen to the atmosphere [2 marks]
    Decomposers (bacteria/fungi): convert organic nitrogen in dead matter to ammonia (ammonification) [2 marks]
    [1 mark for name + 1 mark for correct role; any two pairs]
  • (b) Mean per quadrat: (3+5+2+4+3+6+2+4+5+6) ÷ 10 = 40 ÷ 10 = 4 daisies [1 mark]
    Area of one quadrat = 0.5 × 0.5 = 0.25 m² [1 mark]
    Estimated total = 4 ÷ 0.25 × 200 = 4 × 4 × 200 = 3200 daisies [1 mark]
Paper 4 Style · Carbon cycle + food webExtended

The diagram below represents a simple food web in a pond:

phytoplankton → water fleas → small fish → large fish
phytoplankton → water fleas → large fish

(a) Identify the producer in this food web and explain its role in the carbon cycle. [3 marks]
(b) A disease kills most of the small fish. Predict the short-term effect on (i) large fish and (ii) water fleas. Explain your answers. [4 marks]

(a) [3 marks]
  • The producer is phytoplankton [1 mark]
  • Phytoplankton carry out photosynthesis, absorbing CO₂ from the water/atmosphere and converting it to organic carbon compounds (glucose, etc.) [1 mark]
  • This transfers carbon from the atmosphere/water into the biosphere (living organisms), forming the basis of the food web [1 mark]
(b) [4 marks]
  • (i) Large fish: population likely to decrease initially [1 mark]; small fish are a food source for large fish — less food available → increased competition among large fish / starvation [1 mark]
  • (ii) Water fleas: population likely to increase initially [1 mark]; small fish eat water fleas — with fewer small fish, less predation pressure on water fleas → water flea population rises [1 mark]
Exam Prep

High-Frequency Mistakes — Topic 19 Overall

Topic 19 exam strategy

Highest-yield Core items: the six ecosystem key terms (especially community vs ecosystem); food chain/web arrows direction (prey to predator); three types of pyramid and which can/cannot be inverted (energy pyramid always upright); why energy decreases at each trophic level (heat from respiration + faeces); limiting factors (biotic and abiotic); quadrat sampling calculation (mean per quadrat → scale up to total area). For Extended: carbon cycle process table (photosynthesis/respiration/combustion/decomposition); nitrogen cycle five processes (fixation/nitrification/assimilation/ammonification/denitrification — match each to the correct bacterial species); mark-recapture formula N = (n₁ × n₂) ÷ m₂ and its four assumptions. Nitrogen cycle and mark-recapture are near-certain Paper 4 targets.

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