IGCSE Biology · Topic 13 · 2026 Exam

Excretion in Humans

Definition of excretion and its distinction from egestion; the three organs of excretion; urea production by deamination in the liver; and Extended: kidney structure and nephron, ultrafiltration mechanism, selective reabsorption of glucose and amino acids, ADH and osmoregulation feedback loop, and kidney dialysis.

Sub-section 13.1 Core Extended Papers 1–4
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Topic 13.1

Excretion in Humans

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What is Excretion?

Syllabus definition

Excretion is the removal from the body of the waste products of metabolism.

Excretion vs egestion — the most common Topic 13 error

Excretion removes metabolic waste — substances produced inside body cells as by-products of chemical reactions: CO₂ from respiration, urea from amino acid breakdown, water from metabolic reactions.

Egestion removes undigested food (faeces) through the anus — this material has never been metabolised inside cells and is therefore not excretion.

Organs of Excretion

OrganWaste excretedHow it is produced
Lungs Carbon dioxide; water vapour CO₂ and H₂O are produced by aerobic respiration in every body cell; transported in blood to lungs and exhaled
Kidneys Urea; excess water; excess mineral ions Urea is produced in the liver from excess amino acids; kidneys filter blood and produce urine
Skin Water; mineral ions; small amount of urea Released in sweat by sweat glands; primarily for temperature regulation, but the products are also metabolic waste

Urea Production — Deamination

Proteins cannot be stored in the body. When more amino acids are consumed than needed for protein synthesis, the excess must be broken down. This happens in the liver:

Deamination — two-step process

Step 1 — Deamination: The amino group (–NH₂) is removed from excess amino acids, producing ammonia (NH₃) and an organic acid (keto acid). This occurs in liver cells.

Step 2 — Urea formation: Ammonia is highly toxic, so it is immediately converted to the less toxic compound urea (CO(NH₂)₂) in the liver via the urea cycle.

Urea is then released into the blood, transported to the kidneys, filtered out, and excreted in urine.

SubstanceWhere producedWhy dangerous if not removedHow excreted
UreaLiver (from deamination of excess amino acids)Toxic at high concentrations; disrupts cell functionDissolved in urine via kidneys
Carbon dioxideAll body cells (aerobic respiration)Forms carbonic acid in blood → lowers pH → enzyme denaturationExhaled via lungs

Kidney Structure — Extended

StructureLocationFunction
CortexOuter region of kidneyContains the Bowman's capsules and glomeruli (site of ultrafiltration); also contains proximal and distal convoluted tubules
MedullaInner region of kidneyContains the loops of Henlé and collecting ducts; involved in concentrating urine
PelvisCentral funnel-shaped cavityCollects urine from all nephrons and channels it into the ureter
UreterTube from kidney to bladderCarries urine from kidney to bladder
NephronMicroscopic tubule (each kidney has ~1 million)Functional unit of the kidney — filters blood and produces urine by ultrafiltration and selective reabsorption

Ultrafiltration — Extended

Ultrafiltration — how it works

Location: Bowman's capsule (in the cortex)

Mechanism:

1. Blood enters the glomerulus (a knot of capillaries) via the afferent arteriole. The afferent arteriole is wider than the efferent arteriole — this creates high pressure in the glomerulus.

2. The high pressure forces small molecules from the blood plasma through the capillary walls and into the Bowman's capsule, forming the glomerular filtrate.

3. Molecules small enough to be filtered: water, glucose, amino acids, urea, mineral ions, small drugs.

4. Molecules too large to be filtered (remain in blood): red blood cells, white blood cells, platelets, plasma proteins (e.g. albumin).

Blood cells and proteins should NOT appear in urine

Their presence in urine (haematuria, proteinuria) indicates damage to the glomerular filtration barrier — a sign of kidney disease. This is tested in Paper 4 data questions.

Selective Reabsorption — Extended

The glomerular filtrate flows along the nephron tubule. Useful substances are reclaimed back into the blood by selective reabsorption:

SubstanceReabsorbed?Where & how
All glucose✓ Completely (normally)Proximal convoluted tubule — by active transport (against concentration gradient; requires ATP)
All amino acids✓ CompletelyProximal convoluted tubule — active transport
Water✓ Most (~99%)Proximal tubule, loop of Henlé, collecting duct — by osmosis; amount regulated by ADH
Mineral ions✓ Most (variable)Proximal and distal tubules — active transport; regulated by hormones
Urea✗ Not reabsorbed (remains in filtrate)Passes along the entire nephron and is excreted in urine
Why glucose requires active transport for reabsorption

By the time filtrate reaches the proximal convoluted tubule, the glucose concentration in the tubule lumen may still be high. Active transport (using ATP from mitochondria-rich tubule cells) moves glucose against its concentration gradient back into the surrounding capillaries. This is why tubule cells are packed with mitochondria.

Osmoregulation and ADH — Extended

The ADH feedback loop

Osmoregulation = the regulation of water content (and therefore solute concentration) of body fluids.

When blood water content is too LOW (e.g. dehydration, sweating):

→ Osmoreceptors in the hypothalamus detect the lower water potential

→ The pituitary gland releases more ADH (antidiuretic hormone) into the blood

→ ADH makes the collecting duct walls more permeable to water

→ More water is reabsorbed from the collecting duct back into blood by osmosis

Small volume of concentrated (dark) urine is produced

→ Blood water content rises back to normal (negative feedback)

When blood water content is too HIGH (e.g. drinking large amounts of water):

→ Less ADH released → collecting duct less permeable → less water reabsorbed → large volume of dilute (pale) urine

ConditionADH levelCollecting duct permeabilityUrine produced
Dehydrated / hot / exercisingHighHigh (more permeable)Small volume, concentrated, dark
Well-hydrated / cold / at restLowLow (less permeable)Large volume, dilute, pale

Kidney Failure and Dialysis — Extended

If the kidneys fail, urea and other waste products accumulate in the blood to dangerous levels. Two treatment options:

Kidney dialysis

Blood is passed through a machine containing a partially permeable membrane surrounded by dialysis fluid. Urea and excess ions diffuse out of the blood across the membrane into the dialysis fluid (down concentration gradient). Glucose and useful ions are kept at the correct concentration in the dialysis fluid so they are not lost from the blood. The patient requires dialysis several times a week for several hours.

Kidney transplant

A donor kidney (from a living or deceased donor) is surgically implanted. It must be closely matched in tissue type to reduce the risk of rejection. The patient takes immunosuppressant drugs for life to prevent the immune system attacking the donor kidney. A successful transplant restores normal kidney function and removes the need for dialysis.

Why dialysis fluid contains glucose

Dialysis fluid contains glucose at normal blood concentration. If the fluid had no glucose, glucose would diffuse out of the blood down its concentration gradient — the patient would lose vital glucose. By matching blood glucose concentration in the dialysis fluid, no net movement of glucose occurs, so it is conserved.

Paper 4 Style · Topic 13.1Extended

On a hot day, a person exercises heavily and does not drink. Describe and explain the changes in ADH level and urine production that would occur. [4 marks]

Mark scheme
  • Sweating causes loss of water from blood → blood water potential decreases (blood becomes more concentrated) [1 mark]
  • Osmoreceptors in the hypothalamus detect this → more ADH is released from the pituitary gland [1 mark]
  • ADH makes the collecting duct walls more permeable to water → more water is reabsorbed by osmosis back into blood [1 mark]
  • Result: a small volume of concentrated (dark) urine is produced; blood water content is restored toward normal [1 mark]
MCQ · Topic 13.1Core

Which row correctly identifies where urea is produced and where it is excreted?

Produced inExcreted by
AKidneysLiver
BLiverLungs
CLiverKidneys
DKidneysSkin
Answer: C. Urea is produced in the liver by deamination of excess amino acids. It is then transported in the blood to the kidneys, where it is filtered out and excreted in urine. A common trap is confusing the site of production (liver) with the site of excretion (kidneys).
Exam Prep

Comprehensive Practice Questions

Mixed questions across Topic 13.

MCQ · Excretion definitionCore

Which of the following is an example of excretion?

  • A. Undigested food passing out of the anus as faeces
  • B. Carbon dioxide leaving the body through the lungs during exhalation
  • C. Food being broken down by digestive enzymes in the small intestine
  • D. Water being absorbed from the large intestine into the blood
Answer: B. Carbon dioxide is a metabolic waste product of aerobic respiration — it is produced inside body cells and must be removed. Exhaling CO₂ is therefore excretion. (A) is egestion — undigested food was never metabolised. (C) is digestion. (D) is absorption.
Paper 3 Style · Deamination + kidneysCore

(a) Define excretion. [1 mark]
(b) Explain why the body must remove urea. Include how urea is produced. [3 marks]
(c) State two other substances excreted by the kidneys. [2 marks]

Mark scheme
  • (a) Excretion is the removal from the body of the waste products of metabolism [1 mark]
  • (b) Urea is produced in the liver by deamination of excess amino acids [1 mark]; urea is toxic at high concentrations [1 mark]; if not removed it would accumulate in the blood and disrupt cell function / enzyme activity [1 mark]
  • (c) Any two of: excess water / excess mineral ions [2 marks]
Paper 4 Style · Nephron + dialysisExtended

(a) Explain what happens during ultrafiltration in the Bowman's capsule. State which substances are filtered and which are not. [4 marks]
(b) Explain why dialysis fluid used in kidney machines contains glucose at normal blood concentration but no urea. [3 marks]

(a) [4 marks]
  • The afferent arteriole is wider than the efferent arteriole, creating high blood pressure in the glomerulus [1 mark]
  • High pressure forces small molecules through the capillary wall into the Bowman's capsule, forming the glomerular filtrate [1 mark]
  • Filtered substances: water, glucose, amino acids, urea, mineral ions [1 mark]
  • Not filtered (too large): red blood cells, plasma proteins (e.g. albumin), white blood cells [1 mark]
(b) [3 marks]
  • Dialysis fluid contains no urea so that urea in the blood diffuses out across the partially permeable membrane down its concentration gradient — removing it from the blood [1 mark]
  • Dialysis fluid contains glucose at normal blood concentration so that there is no net diffusion of glucose from blood into the fluid [1 mark]
  • This ensures the patient does not lose glucose (which is needed for respiration) during dialysis treatment [1 mark]
Exam Prep

High-Frequency Mistakes — Topic 13 Overall

Topic 13 exam strategy

Highest-yield Core items: the definition of excretion (metabolic waste — learn word for word); three organs of excretion with their specific products; urea production by deamination in the liver and excretion by kidneys. For Extended: ultrafiltration mechanism (high pressure → small molecules filtered; large molecules retained); selective reabsorption (glucose and amino acids completely reabsorbed by active transport; urea not reabsorbed); the ADH feedback loop (dehydration → more ADH → more permeable collecting duct → concentrated urine); and dialysis fluid composition with reasoning. The ADH mechanism and dialysis fluid question are near-certain Paper 4 targets.

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