IGCSE Biology · Topic 9 · 2026 Exam

Transport in Animals

The mammalian double circulation and its advantages; heart structure, function, and coronary heart disease; the structure–function relationship of arteries, veins, and capillaries; and the four components of blood with their roles — including Extended detail on heart wall thickness, valve action, hepatic portal vein, lymphocytes vs phagocytes, and blood clotting.

Sub-sections 9.1–9.4 Core Extended Papers 1–6
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Topic 9.1

Circulatory Systems

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Core definition

The circulatory system is a system of blood vessels with a pump (the heart) and valves to ensure one-way flow of blood.

Single vs Double Circulation — Extended

FeatureSingle circulation (fish)Double circulation (mammals)
Number of circuitsOne — heart → gills → body → heartTwo — pulmonary (heart ↔ lungs) + systemic (heart ↔ body)
Number of heart chambers2 (one atrium + one ventricle)4 (two atria + two ventricles)
Blood pressure reaching bodyLow — pressure drops after passing through gill capillariesHigh — pressure boosted by left ventricle before reaching body
Blood oxygenation routeDeoxygenated blood pumped from heart to gills; oxygenated blood flows directly to the body before returning deoxygenated to the heart — no separate re-pressurisation circuitOxygenated and deoxygenated blood completely separated by septum; blood re-pressurised by left ventricle after returning from lungs
Advantages of double circulation

1. Higher blood pressure to the body: After blood is oxygenated in the lungs, it returns to the left side of the heart and is pumped again at high pressure to the rest of the body. Fish blood pressure drops after passing through the gill capillaries and reaches the body tissues at low pressure — mammals deliver blood more forcefully.

2. Complete separation of oxygenated and deoxygenated blood: The septum prevents mixing. Tissues always receive fully oxygenated blood, maximising oxygen delivery for aerobic respiration. This supports the high metabolic rate of warm-blooded mammals.

Topic 9.2

The Heart

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Heart Structures — Core

StructureDescription and function
Left and right atriaUpper chambers; receive blood returning to the heart (from veins); thin walls — only pump blood a short distance into the ventricles below
Left and right ventriclesLower chambers; pump blood out of the heart; thick muscular walls to generate high pressure
SeptumMuscular wall separating the left and right sides of the heart; prevents mixing of oxygenated (left) and deoxygenated (right) blood
Muscular wallCardiac muscle adapted for continuous rhythmic contraction; highly resistant to fatigue; generates the pumping force
One-way valvesPrevent backflow of blood; ensure blood flows in one direction only
Coronary arteriesSupply oxygen and nutrients to the heart muscle itself; branch off the aorta just above the heart
The blood flow route through the heart

Right side (deoxygenated blood): vena cava → right atrium → right ventricle → pulmonary artery → lungs

Left side (oxygenated blood): pulmonary vein → left atrium → left ventricle → aorta → body

Memory: blood always enters atria, exits through ventricles. Right side = pulmonary circuit (to lungs). Left side = systemic circuit (to body).

Valve Types — Extended

ValveLocationOpens when…Closes when…
Atrioventricular (AV) valvesBetween each atrium and ventricleVentricle relaxes (atrium pressure > ventricle)Ventricle contracts (prevents backflow into atria)
Semilunar (SL) valvesAt the base of aorta and pulmonary arteryVentricle contracts (ventricle pressure > artery)Ventricle relaxes (prevents backflow from arteries)

Wall Thickness Explained — Extended

ComparisonWhich is thicker?Reason
Left vs right ventricle Left ventricle is thicker Left ventricle pumps blood through the entire systemic circulation (all body organs) — a much longer route at higher pressure. Right ventricle only pumps to the nearby lungs — shorter distance, lower pressure needed.
Atria vs ventricles Ventricles are thicker Atria only push blood into the ventricles directly below — a short, low-resistance path. Ventricles must pump blood out of the heart through arteries against greater resistance — much higher force needed.

How the Heart Beats — Extended

The heart beat follows a coordinated sequence:

  • Both atria contract simultaneously → blood pushed into ventricles; AV valves open
  • Both ventricles contract simultaneously → blood pushed into arteries; SL valves open; AV valves close (prevents backflow)
  • Both ventricles relax → SL valves close (blood in arteries cannot flow back); atria refill from veins
  • Cycle repeats: ~70 times per minute at rest

Monitoring Heart Activity — Core

ECG (electrocardiogram)

Records the electrical activity of the heart as a trace. Normal pattern shows regular peaks; abnormal patterns indicate irregular heartbeat or damage.

Pulse rate

Each heartbeat produces a pressure wave felt at pulse points (wrist, neck). Pulse rate = heart rate. Measured as beats per minute.

Listening to valve sounds

A stethoscope detects the “lub-dub” sounds of the AV and SL valves closing. Abnormal sounds may indicate faulty valves.

Coronary Heart Disease (CHD)

CHD occurs when coronary arteries become blocked by fatty deposits (atherosclerosis), reducing blood flow to the heart muscle. If a coronary artery is completely blocked, the heart muscle it supplies dies — a heart attack (myocardial infarction).

Risk factorHow it contributes
DietHigh saturated fat → increased LDL cholesterol → deposits in artery walls
Lack of exerciseWeak heart muscle; high blood pressure; obesity risk; poor cholesterol profile
StressRaises blood pressure; may lead to unhealthy behaviours (smoking, poor diet)
SmokingNicotine raises heart rate and blood pressure; carbon monoxide reduces O₂ in blood; chemicals damage artery walls
Genetic predispositionFamily history increases risk regardless of lifestyle factors
AgeArteries stiffen and narrow with age; deposits accumulate over time
SexMales at higher risk, especially below age 50; female hormones (oestrogen) offer some protection pre-menopause
Reducing CHD risk through diet and exercise

Diet: Reduce saturated fat (lowers LDL cholesterol); increase fruit, vegetables, fibre; maintain healthy body weight; reduce salt (lowers blood pressure).

Exercise: Strengthens heart muscle; lowers resting heart rate; reduces blood pressure; raises HDL (“good”) cholesterol; reduces obesity risk. Regular aerobic exercise (e.g. 150 min/week moderate intensity) significantly reduces CHD risk.

MCQ · Topic 9.2Core

Why is the left ventricle wall thicker than the right ventricle wall?

  • A. The left ventricle receives more blood than the right
  • B. The left ventricle pumps blood to the lungs, which are closer
  • C. The left ventricle must generate higher pressure to pump blood around the whole body
  • D. The right ventricle is thinner because it pumps oxygenated blood
Answer: C. The left ventricle pumps blood through the entire systemic circulation — to the brain, limbs, organs, and back. This requires much higher pressure than the right ventricle, which only pumps blood the short distance to the lungs (pulmonary circulation). Thicker muscle generates higher pressure. Both ventricles pump equal volumes of blood per beat — only the pressure (and distance) differs.
Topic 9.3

Blood Vessels

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Artery, Vein, Capillary — Comparison

FeatureArteryVeinCapillary
Wall thicknessThick, muscular, elasticThinner than arteryOne cell thick (endothelium only)
Lumen diameterNarrow relative to wallWide relative to wallVery narrow — barely fits one RBC
Valves?None (except at heart)✓ Present throughout — prevent backflowNone
Blood pressureHigh, pulsatileLow, steadyVery low
Direction of flowAway from heartToward heartLinks arterioles to venules
Functions of capillaries

Capillaries are the site of exchange between blood and tissues. Their thin walls (one cell thick) allow oxygen, glucose, and other nutrients to diffuse out into tissue fluid, and CO₂ and waste products to diffuse in from cells. Their narrow lumen slows blood flow, maximising exchange time.

Structure–Function Links — Extended

VesselStructural featureFunctional explanation
Artery Thick muscular and elastic wall Muscle resists high-pressure blood flow without bursting; elastic fibres stretch as blood surges with each heartbeat (systole) and recoil to maintain flow during relaxation (diastole) — smoothing out the pulse
Vein Thin wall; wide lumen; valves Blood in veins is at low pressure — does not need thick walls. Wide lumen reduces resistance to flow. Valves (supported by skeletal muscle contractions) prevent backflow of slow-moving blood returning to the heart
Capillary Wall one cell thick; very small lumen One cell thick = very short diffusion distance for rapid gas and nutrient exchange. Small lumen slows blood, allowing time for exchange. Huge number of capillaries creates enormous total surface area

Named Blood Vessels — Core

VesselConnectsCarries
Vena cavaBody → right atriumDeoxygenated blood from body
AortaLeft ventricle → bodyOxygenated blood to body
Pulmonary arteryRight ventricle → lungsDeoxygenated blood to lungs
Pulmonary veinLungs → left atriumOxygenated blood from lungs
Renal arteryAorta → kidneyOxygenated blood to kidney
Renal veinKidney → vena cavaDeoxygenated blood from kidney
Hepatic arteryAorta → liverOxygenated blood to liver
Hepatic veinLiver → vena cavaDeoxygenated blood from liver
Hepatic portal veinSmall intestine → liverNutrient-rich blood (absorbed from gut) — neither fully oxygenated nor deoxygenated
Hepatic portal vein — Extended high-frequency trap

The hepatic portal vein is unique: it carries blood rich in absorbed nutrients (glucose, amino acids) directly from the small intestine to the liver before they reach the general circulation. The liver processes these nutrients (e.g. converts excess glucose to glycogen, deaminates excess amino acids). This vessel is very frequently tested in Extended papers.

MCQ · Topic 9.3Core

Which blood vessel carries oxygenated blood away from the heart to the body?

  • A. Vena cava
  • B. Pulmonary artery
  • C. Pulmonary vein
  • D. Aorta
Answer: D — Aorta. The aorta leaves the left ventricle and carries freshly oxygenated blood to the body. Common traps: the pulmonary artery (B) is an artery but carries deoxygenated blood to the lungs; the pulmonary vein (C) carries oxygenated blood but toward the heart, not away; the vena cava (A) returns deoxygenated blood from the body to the heart.
Topic 9.4

Blood

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Components and Functions

ComponentDescriptionFunction(s)
Red blood cells
(erythrocytes)
Biconcave disc; no nucleus; packed with haemoglobin; very flexible Transport oxygen: haemoglobin binds O₂ in lungs (forms oxyhaemoglobin) and releases it in body tissues. No nucleus → more space for haemoglobin. Biconcave shape → larger surface area for O₂ diffusion.
White blood cells
(leucocytes)
Larger than RBCs; have a nucleus; fewer in number; various types Defence against pathogens: phagocytosis and antibody production (see below)
Platelets
(thrombocytes)
Tiny cell fragments; no nucleus Blood clotting — aggregate at wound sites and initiate the clotting cascade (details not required at Core)
Plasma Pale yellow liquid (~55% of blood volume); mainly water Transports: blood cells, ions, nutrients (glucose, amino acids, fatty acids), urea, hormones, CO₂, and plasma proteins (including antibodies and fibrinogen)
Blood clotting — roles

When a blood vessel is damaged, clotting: (1) prevents blood loss by sealing the wound with a clot, and (2) prevents entry of pathogens through the wound. Together these protect the body from blood loss and infection simultaneously.

White Blood Cells: Phagocytes vs Lymphocytes — Extended

Cell typeAppearanceFunctionHow identified in photomicrographs
Phagocytes Irregular lobed nucleus; large cell Engulf and digest pathogens by phagocytosis — the cell membrane surrounds and absorbs the pathogen into a vacuole, where it is digested by enzymes Multi-lobed (irregular) nucleus; granular cytoplasm
Lymphocytes Large, round nucleus almost filling the cell; small amount of cytoplasm Produce antibodies specific to antigens on pathogens; antibodies bind to pathogens and mark them for destruction or directly neutralise them Large spherical nucleus; very little cytoplasm visible

Blood Clotting Mechanism — Extended

The clotting process

When a blood vessel is damaged, platelets aggregate at the wound. A cascade of reactions is triggered, converting the soluble plasma protein fibrinogen into insoluble fibrin threads. These threads form a mesh that traps blood cells and forms a clot (scab), sealing the wound.

Summary: Platelets → clotting cascade → fibrinogen → fibrin mesh → clot

MCQ · Topic 9.4Core

Which component of blood is responsible for transporting urea from the liver to the kidneys?

  • A. Red blood cells
  • B. White blood cells
  • C. Platelets
  • D. Plasma
Answer: D — Plasma. Plasma is the liquid component of blood that transports dissolved substances including urea, glucose, hormones, CO₂, and ions. Red blood cells carry oxygen (as oxyhaemoglobin). White blood cells are involved in immunity. Platelets are involved in clotting. Urea produced by the liver travels dissolved in plasma to the kidneys for excretion.
Paper 4 Style · Topic 9.4Extended

A student observes two types of white blood cell in a photomicrograph: Cell A has a large, round nucleus nearly filling the cell. Cell B has a multi-lobed irregular nucleus.

(a) Identify cells A and B. [2 marks]
(b) Describe how Cell B destroys pathogens. [3 marks]
(c) Describe the process by which Cell A produces a defence response to a specific pathogen. [2 marks]

Mark scheme
  • (a) Cell A: lymphocyte [1 mark]; Cell B: phagocyte [1 mark]
  • (b) Cell B (phagocyte): engulfs the pathogen by phagocytosis [1 mark]; the pathogen is enclosed in a vacuole [1 mark]; digestive enzymes are released into the vacuole and break down the pathogen [1 mark]
  • (c) Cell A (lymphocyte): recognises a specific antigen on the pathogen’s surface [1 mark]; produces specific antibodies that bind to those antigens, leading to destruction or marking of the pathogen [1 mark]
Exam Prep

Comprehensive Practice Questions

Mixed questions across all of Topic 9.

MCQ · Arteries vs veinsCore

Which statement correctly compares the structure of arteries and veins?

  • A. Veins have thicker walls and a narrower lumen than arteries
  • B. Arteries have valves throughout their length; veins do not
  • C. Arteries have thicker walls than veins; veins have valves but arteries do not
  • D. Arteries and veins have the same wall thickness but different lumen diameters
Answer: C. Arteries have thick muscular elastic walls to withstand high blood pressure. Veins have thinner walls (blood pressure is low) and contain valves to prevent backflow. Arteries generally do not have valves along their length (except the semilunar valves at the aorta and pulmonary artery).
Paper 3 Style · Heart + CHDCore

(a) Name the blood vessel that carries deoxygenated blood from the heart to the lungs. [1 mark]
(b) Describe what is meant by coronary heart disease (CHD). [2 marks]
(c) State two lifestyle risk factors for CHD and, for each, explain how it contributes to the disease. [4 marks]

Mark scheme
  • (a) Pulmonary artery [1 mark]
  • (b) CHD is the blockage of coronary arteries [1 mark] by fatty deposits / atherosclerosis, reducing blood (oxygen) supply to the heart muscle [1 mark]
  • (c) Any two lifestyle factors, each with explanation — e.g.:
    Smoking: nicotine raises blood pressure / carbon monoxide reduces O₂ in blood / chemicals damage artery walls → increases atherosclerosis risk [2 marks]
    Diet high in saturated fat: raises LDL (bad) cholesterol in blood → cholesterol deposits in artery walls → arteries narrow → blockage risk [2 marks]
    Lack of exercise: weakens heart; leads to obesity and high blood pressure; poor cholesterol balance [2 marks]
Paper 4 Style · Double circulation + blood vesselsExtended

(a) Explain two advantages of the mammalian double circulation over the single circulation of a fish. [4 marks]
(b) After a meal, blood in the hepatic portal vein has a higher concentration of glucose than blood in the hepatic artery. Explain why, and state what happens to this glucose in the liver. [3 marks]

(a) [4 marks]
  • Advantage 1: Blood is re-pressurised after passing through the lungs [1 mark]; the left ventricle generates high pressure so blood reaches body tissues efficiently with enough force for rapid exchange [1 mark]
  • Advantage 2: Oxygenated and deoxygenated blood are completely separated by the septum [1 mark]; body tissues always receive fully oxygenated blood, maximising oxygen delivery and supporting the high metabolic rate of mammals [1 mark]
(b) [3 marks]
  • The hepatic portal vein carries blood directly from the small intestine to the liver [1 mark]
  • After a meal, glucose absorbed from digested food in the small intestine enters this blood, raising its glucose concentration [1 mark]
  • In the liver, excess glucose is converted to glycogen for storage (glycogenesis), preventing dangerously high blood glucose levels [1 mark]
Exam Prep

High-Frequency Mistakes — Topic 9 Overall

Topic 9 exam strategy

Topic 9 is one of the most content-heavy topics and appears across all papers. Highest-yield items: named blood vessels (especially the oxygenation trick — pulmonary artery carries deoxygenated blood); artery/vein/capillary structure comparison table; why left ventricle is thicker; CHD risk factors with mechanism (not just a list); and blood components with their specific functions. For Extended: double circulation advantages, hepatic portal vein, lymphocyte vs phagocyte identification and function, and the fibrinogen → fibrin clotting mechanism are near-certain Paper 4 targets.

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